abstract：:Pituitary and plasma pools from postmenopausal women and plasma pools from women at midcycle were fractionated by electrofocusing in sucrose density gradients. The biological LH activity was determined in each of the electrofocusing fractions by the use of an in vitro bioassay method. A heterogeneous profile of LH act...

journal_title：Molecular and cellular endocrinology

authors： Robertson DM,Van Damme MP,Diczfalusy E

更新日期：1977-11-01 00:00:00

abstract：:This study examines the effect of FSH, testosterone and estradiol on testicular LH receptors and in vitro testicular responsiveness to LH in immature rats under various conditions. FSH treatment of 15-day-old immature rats significantly increased the number of LH receptors but did not alter testicular responsiveness. ...

journal_title：Molecular and cellular endocrinology

authors： Chen YD,Shaw MJ,Payne AH

更新日期：1977-10-01 00:00:00

abstract：:The metabolism of radioactive testosterone, 5alpha-dihydrotestosterone, 4-androstene-3beta,17beta-diol or 4-androstene-3alpha,17beta-diol by the human cell line NHIK 3025, derived from a carcinoma of the uterine cervix, was studied. The cells were grown in Eagle's minimal essential medium (MEM) with a steriod concentr...

journal_title：Molecular and cellular endocrinology

authors： Stenstad P,Ostgaard K,Eik-nes KB

更新日期：1977-10-01 00:00:00

abstract：:In order to differentiate whether activation of NaK-ATPase in thyroid thermogenesis is due to increased numbers of active 'sodium pump' units or due to a change in the kinetics of the enzyme, the effect of T3 on activation energy (Ea) of NaK-ATPase was determined in rat liver, kidney and brain. Injection of T3 produce...

journal_title：Molecular and cellular endocrinology

authors： Rahimifar M,Ismail-Beigi

更新日期：1977-02-01 00:00:00

abstract：:When measured in vitro using human placental microsomal preparations, the aromatization of 16alpha-hydroxytestosterone to estriol and androstenedione to estrone and estradiol proceeds at almost identical initial rates. Important differences between 16alpha-hydroxytestosterone and adrostenedione aromatization are evide...

journal_title：Molecular and cellular endocrinology

authors： Canick JA,Ryan KJ

更新日期：1976-12-01 00:00:00

abstract：:Determinations of the calcium pools in myocardial cells in vitro have shown the existence of at least three pools of exchangeable calcium. Epinephrine and glucagon were found to produce significant increases in the size of the two slower exchanging pools. Prostaglandins E1 and F1alpha also increased significantly calc...

journal_title：Molecular and cellular endocrinology

authors： Moura AM,Simpkins H

更新日期：1976-10-01 00:00:00

abstract：:The effects of cholera toxin on the responses of cultured Sertoli cells were compared with those elicited by follicle-stimulating hormone (FSH), and N6O2'-dibutyryl-3',5'-cyclic AMP (bu2cAMP). Addition of FSH or cholera toxin increased cAMP levels. Subsequently there was greater rates of conversion of testosterone to ...

journal_title：Molecular and cellular endocrinology

authors： Fritz IB,Griswold MD,Louis BG,Dorrington JH

更新日期：1976-08-01 00:00:00

abstract：:In vitro RNA synthesis has been analyzed using autoradiographic and electrophoretic methods in isolated 1-2 mm segments of rat seminiferous tubules. The cellular composition of each segment was accurately identified using microscopic analysis of the transillumination pattern of the freshly isolated, unstained, seminif...

journal_title：Molecular and cellular endocrinology

authors： Söderström KO,Parvinen M

更新日期：1976-08-01 00:00:00

abstract：:The adenosine 3', 5'-cyclic monophosphate (cyclic AMP)-dependent protein phosphokinase of rat interstitial cells was characterized by ion-exchange chromatography and sucrose density gradient centrifugation. The 0.2 M NaCl fraction from DEAE-Sephadex showed a small 2.9-S peak of basal enzyme activity, and a large 6.5-S...

journal_title：Molecular and cellular endocrinology

authors： Podesta EJ,Dufau ML,Catt KJ

更新日期：1976-06-01 00:00:00

abstract：:The earliest known induction by estrogen of a specific uterine protein is that of the induced protein (IP) of Gorski and colleagues, which is demonstrable within 40 min after treatment. We found that this protein is not restricted to the rat; it was detected i the uterus of prepuberal C3H/eB and SWR mice, 1 h after i...

journal_title：Molecular and cellular endocrinology

authors： Sömjen D,Kaye AM

更新日期：1976-05-01 00:00:00

abstract：:Immature rat testes contain a specific binding protein for testosterone (T) and 5alpha-dihyrotestosterone (DHT) with physico-chemical properties similar to the cytoplasmic androgen receptors in the epididymis and ventral prostate but different from the testicular androgen-binding protein (ABP). Like the androgen recep...

journal_title：Molecular and cellular endocrinology

authors： McLean WS,Smith AA,Hansson V,Naess O,Nayfeh SN,French FS

更新日期：1976-03-01 00:00:00

abstract：:Administration of hydrocortisone to adrenalectomised rats elevated levels of both free and membrane-bound polysomes in liver and led to marked shifts in their sedimentation profiles in favour of heavier polysomes. The rate of nascent polypeptide formation was also found to be enhanced in response to the hormone but th...

journal_title：Molecular and cellular endocrinology

authors： Kulkarni SB,Netrawali MS,Pradhan DS,Sreenivasan A

更新日期：1976-02-01 00:00:00

abstract：:An in vitro method has been developed for the quantitative estimation of juvenile hormone (JH) esterase activity in the hemolymph of the Colorado beetle Leptinotarsa decemlineata Say. The apparent Km value for JH-esterase was determined. JH-esterase and general carboxyesterase activities were estimated throughout the ...

journal_title：Molecular and cellular endocrinology

authors： Kramer SJ,DeKort CA

更新日期：1975-12-01 00:00:00

abstract：:A macromolecular component binding 3H-labelled 17 beta-oestradiol in a specific manner and sedimenting in the 8-10-S region on sucrose gradient has been detected in the mammary gland cytosol of ovariectomized adult virgin mice. The dissociation constant of the macromolecule-oestradiol complex is 4.2 times 10(-10)M at ...

journal_title：Molecular and cellular endocrinology

authors： Auricchio F,Rotondi A,Bresciani F

更新日期：1975-12-01 00:00:00

abstract：:The electrophoretic mobility and surface charge of thymocytes was reduced on incubation of the cells with growth hormone in vitro. All cells in thymus were not found to be sensitive to the hormone. The proportion of cells whose surface charge was altered by the hormone was 65% in the thymocytes from 5-week-old rats. T...

journal_title：Molecular and cellular endocrinology

authors： Hanjan SN,Talwar GP

更新日期：1975-09-01 00:00:00

abstract：:After i.m. injection of [3H]butyrobetaine into rats, the accumulation of carnitine into the epididymis, prostate gland, seminal vesicles, testis and heart was studied. The concentration of radiolabeled carnitine into the cauda epididymis increased linearly with time up to 72 h after the injection of the precursor, whi...

journal_title：Molecular and cellular endocrinology

authors： Bohmer T,Hansson V

更新日期：1975-08-01 00:00:00

abstract：:[3-H]Melatonin administered in vivo in the rat cisterna magna became associated with a vinblastine-precipitable protein. Melatonin treatment decreased microtubule protein content by 44% in the arcuate-median eminence region and by 19% in the remaining hypothalamic block, being without significant effect on the cerebra...

journal_title：Molecular and cellular endocrinology

authors： Cardinali DP,Freire F

更新日期：1975-05-01 00:00:00